Incorporation of (R)- and (S)-3',4'-seco-thymidine into oligodeoxynucleotides: hybridization properties and enzymatic stability.
Identifieur interne : 004280 ( Main/Exploration ); précédent : 004279; suivant : 004281Incorporation of (R)- and (S)-3',4'-seco-thymidine into oligodeoxynucleotides: hybridization properties and enzymatic stability.
Auteurs : P. Nielsen [Danemark] ; F. Kirpekar ; J. WengelSource :
- Nucleic acids research [ 0305-1048 ] ; 1994.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires, Hybridation d'acides nucléiques, Oligodésoxyribonucléotides (), Oligodésoxyribonucléotides (métabolisme), Phosphodiesterase I, Phosphodiesterases (métabolisme), Spectroscopie par résonance magnétique, Stabilité enzymatique, Structure moléculaire, Séquence nucléotidique, Thymidine (), Thymidine (analogues et dérivés), Thymidine (métabolisme).
- MESH :
- analogues et dérivés : Thymidine.
- métabolisme : Oligodésoxyribonucléotides, Phosphodiesterases, Thymidine.
- Données de séquences moléculaires, Hybridation d'acides nucléiques, Oligodésoxyribonucléotides, Phosphodiesterase I, Spectroscopie par résonance magnétique, Stabilité enzymatique, Structure moléculaire, Séquence nucléotidique, Thymidine.
English descriptors
- KwdEn :
- Base Sequence, Enzyme Stability, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Nucleic Acid Hybridization, Oligodeoxyribonucleotides (chemistry), Oligodeoxyribonucleotides (metabolism), Phosphodiesterase I, Phosphoric Diester Hydrolases (metabolism), Thymidine (analogs & derivatives), Thymidine (chemistry), Thymidine (metabolism).
- MESH :
- chemical , analogs & derivatives : Thymidine.
- chemical , chemistry : Oligodeoxyribonucleotides, Thymidine.
- chemical , metabolism : Oligodeoxyribonucleotides, Phosphoric Diester Hydrolases, Thymidine.
- Base Sequence, Enzyme Stability, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Nucleic Acid Hybridization, Phosphodiesterase I.
Abstract
Novel flexible oligodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidite approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications in the middle or one or two modifications in the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modified oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.
DOI: 10.1093/nar/22.5.703
PubMed: 8139908
Affiliations:
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Nielsen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Odense University, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Department of Chemistry, Odense University</wicri:regionArea><wicri:noRegion>Odense University</wicri:noRegion></affiliation></author><author><name sortKey="Kirpekar, F" sort="Kirpekar, F" uniqKey="Kirpekar F" first="F" last="Kirpekar">F. Kirpekar</name></author><author><name sortKey="Wengel, J" sort="Wengel, J" uniqKey="Wengel J" first="J" last="Wengel">J. Wengel</name></author></analytic><series><title level="j">Nucleic acids research</title><idno type="ISSN">0305-1048</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Enzyme Stability</term><term>Magnetic Resonance Spectroscopy</term><term>Molecular Sequence Data</term><term>Molecular Structure</term><term>Nucleic Acid Hybridization</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Oligodeoxyribonucleotides (metabolism)</term><term>Phosphodiesterase I</term><term>Phosphoric Diester Hydrolases (metabolism)</term><term>Thymidine (analogs & derivatives)</term><term>Thymidine (chemistry)</term><term>Thymidine (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Hybridation d'acides nucléiques</term><term>Oligodésoxyribonucléotides ()</term><term>Oligodésoxyribonucléotides (métabolisme)</term><term>Phosphodiesterase I</term><term>Phosphodiesterases (métabolisme)</term><term>Spectroscopie par résonance magnétique</term><term>Stabilité enzymatique</term><term>Structure moléculaire</term><term>Séquence nucléotidique</term><term>Thymidine ()</term><term>Thymidine (analogues et dérivés)</term><term>Thymidine (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Thymidine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Oligodeoxyribonucleotides</term><term>Thymidine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oligodeoxyribonucleotides</term><term>Phosphoric Diester Hydrolases</term><term>Thymidine</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Thymidine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Oligodésoxyribonucléotides</term><term>Phosphodiesterases</term><term>Thymidine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Enzyme Stability</term><term>Magnetic Resonance Spectroscopy</term><term>Molecular Sequence Data</term><term>Molecular Structure</term><term>Nucleic Acid Hybridization</term><term>Phosphodiesterase I</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Hybridation d'acides nucléiques</term><term>Oligodésoxyribonucléotides</term><term>Phosphodiesterase I</term><term>Spectroscopie par résonance magnétique</term><term>Stabilité enzymatique</term><term>Structure moléculaire</term><term>Séquence nucléotidique</term><term>Thymidine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Novel flexible oligodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidite approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications in the middle or one or two modifications in the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modified oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.</div></front></TEI><affiliations><list><country><li>Danemark</li></country></list><tree><noCountry><name sortKey="Kirpekar, F" sort="Kirpekar, F" uniqKey="Kirpekar F" first="F" last="Kirpekar">F. Kirpekar</name><name sortKey="Wengel, J" sort="Wengel, J" uniqKey="Wengel J" first="J" last="Wengel">J. Wengel</name></noCountry><country name="Danemark"><noRegion><name sortKey="Nielsen, P" sort="Nielsen, P" uniqKey="Nielsen P" first="P" last="Nielsen">P. Nielsen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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